Digital PCR improves the quantitation of DMR and the selection of CML candidates to TKIs discontinuation.

ABSTRACT: Treatment-free remission (TFR) by tyrosine kinase inhibitors (TKI) discontinuation in patients with deep molecular response (DMR) is a paramount goal in the current chronic myeloid leukemia (CML) therapeutic strategy. The best DMR level by real-time quantitative PCR (RT-qPCR) for TKI discontinuation is still a matter of debate. To compare the accuracy of digital PCR (dPCR) and RT-qPCR for BCR-ABL1 transcript levels detection, 142 CML patients were monitored for a median time of 24 months. Digital PCR detected BCR-ABL1 transcripts in the RT-qPCR undetectable cases. The dPCR analysis of the samples, grouped by the MR classes, revealed a significant difference between MR^4.0 and MR^4.5 (P = 0.0104) or MR^5.0 (P = 0.0032). The clinical and hematological characteri

SUBMITTER: Bernardi S 

PROVIDER: S-EPMC6536984 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature