Dynamic changes to lipid mediators support transitions among macrophage subtypes during muscle regeneration.

ABSTRACT: Muscle damage elicits a sterile immune response that facilitates complete regeneration. Here, we used mass spectrometry-based lipidomics to map the mediator lipidome during the transition from inflammation to resolution and regeneration in skeletal muscle injury. We observed temporal regulation of glycerophospholipids and production of pro-inflammatory lipid mediators (for example, leukotrienes and prostaglandins) and specialized pro-resolving lipid mediators (for example, resolvins and lipoxins) that were modulated by ibuprofen. These time-dependent profiles were recapitulated in sorted neutrophils and Ly6C^hi and Ly6C^lo muscle-infiltrating macrophages, with a distinct pro-resolving signature observed in Ly6C^lo macrophages. RNA sequencing of macrophages sti

SUBMITTER: Giannakis N 

PROVIDER: S-EPMC6537107 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature