TSA-CRAFT: A Free Software for Automatic and Robust Thermal Shift Assay Data Analysis.

ABSTRACT: Thermal shift assay (TSA) is an increasingly popular technique used for identifying protein stabilizing conditions or interacting ligands in X-ray crystallography and drug discovery applications. Although the setting up and running of TSA reactions is a relatively simple process, the subsequent analysis of TSA data, especially in high-throughput format, requires substantial amount of effort if conducted manually. We therefore developed the Thermal Shift Assay-Curve Rapid and Automatic Fitting Tool (TSA-CRAFT), a freely available software that enable automatic analysis of TSA data of any throughput. TSA-CRAFT directly reads real-time PCR instrument data files and displays the analyzed results in a web browser. This software features streamlined data processing and Boltzmann equation fitting

SUBMITTER: Lee PH 

PROVIDER: S-EPMC6537141 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature