Surface sterilization methods impact measures of internal microbial diversity in ticks.

ABSTRACT:

Background

Ticks are obligate blood feeders transmitting major pathogens worldwide. Over the past few years, considerable research efforts have focused on the diversity, distribution and impact of gut and intracellular bacterial symbionts on tick development and tick-borne pathogen transmission. The study of this internal microbiome requires the use of a sterilization method to remove external (i.e. cuticular) microbes present on the tick's surface and to avoid any further contamination. Several sterilization methods exist, including ethanol- or bleach-based treatments that are both effective in killing microbes but with different potential effects on DNA denaturation.

Methods

We examined how these different sterilization methods impact the measure of internal microbial dive

SUBMITTER: Binetruy F 

PROVIDER: S-EPMC6537145 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature