Second generation of pepino mosaic virus vectors: improved stability in tomato and a wide range of reporter genes.

ABSTRACT:

Background

Vectors based on plant viruses are important tools for functional genomics, cellular biology, plant genome engineering and molecular farming. We previously reported on the construction of PepGFP2a, a viral vector based on pepino mosaic virus (PepMV) which expressed GFP efficiently and stably in plants of its experimental host Nicotiana benthamiana, but not in its natural host tomato. We have prepared a new set of PepMV-based vectors with improved stability that are able to express a wide range of reporter genes, useful for both N. benthamiana and tomato.

Results

We first tested PepGFPm1 and PepGFPm2, two variants of PepGFP2a in which we progressively reduced a duplication of nucleotides encoding the N-terminal region of the coat protein. The new vect

SUBMITTER: Ruiz-Ramon F 

PROVIDER: S-EPMC6537163 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature