Fluorescence-based tracing of transplanted intestinal epithelial cells using confocal laser endomicroscopy.

ABSTRACT:

Background

Intestinal stem cell transplantation has been shown to promote mucosal healing and to engender fully functional epithelium in experimental colitis. Hence, stem cell therapies may provide an innovative approach to accomplish mucosal healing in patients with debilitating conditions such as inflammatory bowel disease. However, an approach to label and trace transplanted cells, in order to assess engraftment efficiency and to monitor wound healing, is a key hurdle to overcome prior to initiating human studies. Genetic engineering is commonly employed in animal studies, but may be problematic in humans due to potential off-target and long-term adverse effects.

Methods

We investigated the applicability of a panel of fluorescent dyes and nanoparticles to label intestinal

SUBMITTER: Bergenheim F 

PROVIDER: S-EPMC6537188 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature