MicroRNA-340-5p suppresses non-small cell lung cancer cell growth and metastasis by targeting ZNF503.

ABSTRACT:

Background

MicroRNAs (miRNAs) have been reported to play crucial roles in cancer cell processes, including proliferation, metastasis and cell cycle progression. We aimed to identify miRNAs that could act as suppressors of cell growth and invasion in non-small cell lung cancer (NSCLC).

Methods

Fifteen paired NSCLC tissue samples and pericarcinomatous normal tissues were collected and preserved in liquid nitrogen. The expression levels of miR-340-5p and ZNF503 mRNA were detected using a qPCR assay. The transfection of plasmids was conducted using Lipofectamine 3000 according to the manufacturer's protocol. Cell proliferation was determined using a CCK-8 assay. The protein levels of endothelial-mesenchymal transition markers were measured using a western blot assay. Cell invasi

SUBMITTER: Lu G 

PROVIDER: S-EPMC6537386 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature