An image-based assay to quantify changes in proliferation and viability upon drug treatment in 3D microenvironments.

ABSTRACT:

Background

Every biological experiment requires a choice of throughput balanced against physiological relevance. Most primary drug screens neglect critical parameters such as microenvironmental conditions, cell-cell heterogeneity, and specific readouts of cell fate for the sake of throughput.

Methods

Here we describe a methodology to quantify proliferation and viability of single cells in 3D culture conditions by leveraging automated microscopy and image analysis to facilitate reliable and high-throughput measurements. We detail experimental conditions that can be adjusted to increase either throughput or robustness of the assay, and we provide a stand alone image analysis program for users who wish to implement this 3D drug screening assay in high throughput.

Results

SUBMITTER: Murali VS 

PROVIDER: S-EPMC6537405 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature