Programmable design of orthogonal protein heterodimers.

ABSTRACT: Specificity of interactions between two DNA strands, or between protein and DNA, is often achieved by varying bases or side chains coming off the DNA or protein backbone-for example, the bases participating in Watson-Crick pairing in the double helix, or the side chains contacting DNA in TALEN-DNA complexes. By contrast, specificity of protein-protein interactions usually involves backbone shape complementarity¹, which is less modular and hence harder to generalize. Coiled-coil heterodimers are an exception, but the restricted geometry of interactions across the heterodimer interface (primarily at the heptad a and d positions²) limits the number of orthogonal pairs that can be created simply by varying side-chain interactions^3,4. Here we show that protein-p

SUBMITTER: Chen Z 

PROVIDER: S-EPMC6537907 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature