Transcriptional changes after herpes simplex virus type 1 infection in human trabecular meshwork cells.

ABSTRACT:

Background

Herpes simplex virus type 1 (HSV-1) is causative for hypertensive anterior uveitis. Trabecular meshwork (TM) cells, which are the key cells regulating intraocular pressure (IOP), is considered to be the site of inflammation. We explored the profiles of genes expressed in human TM primary cells upon HSV-1 infection.

Methods

Human TM cells were infected with HSV-1 and total RNA was isolated. The global transcriptional gene network analyses were performed in mock-infected and HSV-1 infected TM cells. Using ingenuity pathway analysis, we determined the key biological networks upon HSV-1 infection. The results of microarray analyses were validated using quantitative PCR.

Results

TM cells had a high susceptibility to HSV-1 infection. HSV-1 induced transcriptional

SUBMITTER: Choi JA 

PROVIDER: S-EPMC6538180 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature