Single cell RNA sequencing identifies TGFβ as a key regenerative cue following LPS-induced lung injury.

ABSTRACT: Many lung diseases result from a failure of efficient regeneration of damaged alveolar epithelial cells (AECs) after lung injury. During regeneration, AEC2s proliferate to replace lost cells, after which proliferation halts and some AEC2s transdifferentiate into AEC1s to restore normal alveolar structure and function. Although the mechanisms underlying AEC2 proliferation have been studied, the mechanisms responsible for halting proliferation and inducing transdifferentiation are poorly understood. To identify candidate signaling pathways responsible for halting proliferation and inducing transdifferentiation, we performed single cell RNA sequencing on AEC2s during regeneration in a murine model of lung injury induced by intratracheal LPS. Unsupervised clustering revealed distinct subpopula

SUBMITTER: Riemondy KA 

PROVIDER: S-EPMC6538357 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature