DNA-encoded bispecific T cell engagers and antibodies present long-term antitumor activity.

ABSTRACT: Specific antibody therapy, including mAbs and bispecific T cell engagers (BiTEs), are important new tools for cancer immunotherapy. However, these approaches are slow to develop and may be limited in their production, thus restricting the patients who can access these treatments. BiTEs exhibit a particularly short half-life and difficult production. The development of an approach allowing simplified development, delivery, and in vivo production would be an important advance. Here we describe the development of a designed synthetic DNA plasmid, which we optimized to permit high expression of an anti-HER2 antibody (HER2dMAb) and delivered it into animals through adaptive electroporation. HER2dMAb was efficiently expressed in vitro and in vivo, reaching levels of 50 μg/ml in mouse sera. Mecha

SUBMITTER: Perales-Puchalt A 

PROVIDER: S-EPMC6538381 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature