Validation and clinical application of a method to quantify nevirapine in dried blood spots and dried breast-milk spots.

ABSTRACT:

Objectives

The validation and clinical application of an LC-MS/MS method for the quantification of nevirapine in dried blood spots (DBS) and dried breast-milk spots (DBMS) are presented.

Methods

DBS and DBMS were prepared from 50 and 30 μL of nevirapine-spiked whole blood and human breast milk, respectively. Chromatographic separation was achieved on a reverse-phase C18 column with 0.1% formic acid in water/acetonitrile using a solvent gradient programme at a flow rate of 400 μL/min, and detection was by a TSQ Quantum Access triple quadrupole mass spectrometer. The clinical application was evaluated in HIV-positive nursing mothers and their breastfed infants.

Results

The assay was validated over the concentration range 50-10,000 ng/mL. Accuracy ranged from 93.3% to 11

SUBMITTER: Olagunju A 

PROVIDER: S-EPMC6538530 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature