Conventionally used reference genes are not outstanding for normalization of gene expression in human cancer research.

ABSTRACT:

Background

The selection of reference genes is essential for quantifying gene expression. Theoretically they should be expressed stably and not regulated by experimental or pathological conditions. However, identification and validation of reference genes for human cancer research are still being regarded as a critical point, because cancerous tissues often represent genetic instability and heterogeneity. Recent pan-cancer studies have demonstrated the importance of the appropriate selection of reference genes for use as internal controls for the normalization of gene expression; however, no stably expressed, consensus reference genes valid for a range of different human cancers have yet been identified.

Results

In the present study, we used large-scale cancer gene expressio

SUBMITTER: Jo J 

PROVIDER: S-EPMC6538551 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature