An electrochemiluminescence based assay for quantitative detection of endogenous and exogenously applied MeCP2 protein variants.

ABSTRACT: Methyl-CpG-binding protein 2 (MeCP2) is a multifunctional chromosomal protein that plays a key role in the central nervous system. Its levels need to be tightly regulated, as both deficiency and excess of the protein can lead to severe neuronal dysfunction. Loss-of-function mutations affecting MeCP2 are the primary cause of Rett syndrome (RTT), a severe neurological disorder that is thought to result from absence of functional protein in the brain. Several therapeutic strategies for the treatment of RTT are currently being developed. One of them is the use of stable and native TAT-MeCP2 fusion proteins to replenish its levels in neurons after permeation across the blood-brain barrier (BBB). Here we describe the expression and purification of various transactivator of transcription (TAT)-Me

SUBMITTER: Steinkellner H 

PROVIDER: S-EPMC6538716 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature