Low frequency Raman Spectroscopy for micron-scale and in vivo characterization of elemental sulfur in microbial samples.

ABSTRACT: Elemental sulfur (S(0)) is an important intermediate of the sulfur cycle and is generated by chemical and biological sulfide oxidation. Raman spectromicroscopy can be applied to environmental samples for the detection of S(0), as a practical non-destructive micron-scale method for use on wet material and living cells. Technical advances in filter materials enable the acquisition of ultra-low frequency (ULF) Raman measurements in the 10-100 cm^-1 range using a single-stage spectrometer. Here we demonstrate the potency of ULF Raman spectromicroscopy to harness the external vibrational modes of previously unrecognized S(0) structures present in environmental samples. We investigate the chemical and structural nature of intracellular S(0) granules stored within environmental mats of

SUBMITTER: Nims C 

PROVIDER: S-EPMC6538736 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature