Lactate Metabolism Is Strongly Modulated by Fecal Inoculum, pH, and Retention Time in PolyFermS Continuous Colonic Fermentation Models Mimicking Young Infant Proximal Colon.

ABSTRACT: The metabolism of lactate impacts infant gut health and may lead to acute accumulation of lactate and/or H₂ associated with pain and crying of colicky infants. Because gut microbiota studies are limited due to ethical and safety concerns, in vitro fermentation models were developed as powerful tools to assess effects of environmental conditions on the gut microbiota. In this study, we established a continuous colonic fermentation model (PolyFermS), inoculated with immobilized fecal microbiota and mimicking the proximal colon of 2-month-old infants. We investigated the effects of pH and retention time (RT) on lactate metabolism and of lactate-utilizing bacteria (LUB) exhibiting little or no H₂ production. We observed that a drop in pH from 6.0 to 5.0 increased t

SUBMITTER: Pham VT 

PROVIDER: S-EPMC6538849 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature