Marek's Disease Virus Regulates the Ubiquitylome of Chicken CD4⁺ T Cells to Promote Tumorigenesis.

ABSTRACT: Ubiquitination and deubiquitination of cellular proteins are reciprocal reactions catalyzed by ubiquitination-related enzymes and deubiquitinase (DUB) which regulate almost all cellular processes. Marek's disease virus (MDV) encodes a viral DUB that plays an important role in the MDV pathogenicity. Chicken CD4⁺ T-cell lymphoma induced by MDV is a key contributor to multiple visceral tumors and immunosuppression of chickens with Marek's disease (MD). However, alterations in the ubiquitylome of MDV-induced T lymphoma cells are still unclear. In this study, a specific antibody against K-ε-GG was used to isolate ubiquitinated peptides from CD4⁺ T cells and MD T lymphoma cells. Mass spectrometry was used to compare and analyze alterations in the ubiquitylome. Our results s

SUBMITTER: Zhou X 

PROVIDER: S-EPMC6539122 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature