Rapid Discovery of Illuminating Peptides for Instant Detection of Opioids in Blood and Body Fluids.

ABSTRACT: The United States is currently experiencing an opioid crisis, with more than 47,000 deaths in 2017 due to opioid overdoses. Current approaches for opioid identification and quantification in body fluids include immunoassays and chromatographic methods (e.g., LC-MS, GC-MS), which require expensive instrumentation and extensive sample preparation. Our aim was to develop a portable point-of-care device that can be used for the instant detection of opioids in body fluids. Here, we reported the development of a morphine-sensitive fluorescence-based sensor chip to sensitively detect morphine in the blood using a homogeneous immunoassay without any washing steps. Morphine-sensitive illuminating peptides were identified using a high throughput one-bead one-compound (OBOC) combinatorial peptide lib

SUBMITTER: Jafari S 

PROVIDER: S-EPMC6539258 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature