Development-Related miRNA Expression and Target Regulation during Staggered In Vitro Plant Regeneration of Tuxpeno VS-535 Maize Cultivar.

ABSTRACT: In vitro plant regeneration addresses basic questions of molecular reprogramming in the absence of embryonic positional cues. The process is highly dependent on the genotype and explant characteristics. However, the regulatory mechanisms operating during organ differentiation from in vitro cultures remain largely unknown. Recently, miRNAs have emerged as key regulators during embryogenic callus induction, plant differentiation, auxin responses and totipotency. Here, we explored how development-related miRNA switches the impact on their target regulation depending on physiological and molecular events taking place during maize Tuxpeño VS-535 in vitro plant regeneration. Three callus types with distinctive regeneration potential were characterized by microscopy and histological preparations.

SUBMITTER: Lopez-Ruiz BA 

PROVIDER: S-EPMC6539278 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature