Ouabain Enhances Cell-Cell Adhesion Mediated by β₁ Subunits of the Na⁺,K⁺-ATPase in CHO Fibroblasts.

ABSTRACT: Adhesion is a crucial characteristic of epithelial cells to form barriers to pathogens and toxic substances from the environment. Epithelial cells attach to each other using intercellular junctions on the lateral membrane, including tight and adherent junctions, as well as the Na⁺,K⁺-ATPase. Our group has shown that non-adherent chinese hamster ovary (CHO) cells transfected with the canine β₁ subunit become adhesive, and those homotypic interactions amongst β₁ subunits of the Na⁺,K⁺-ATPase occur between neighboring epithelial cells. Ouabain, a cardiotonic steroid, binds to the α subunit of the Na⁺,K⁺-ATPase, inhibits the pump activity and induces the detachment of epithelial cells when used at concentra

SUBMITTER: Vilchis-Nestor CA 

PROVIDER: S-EPMC6539428 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature