Changes of Circulating Extracellular Vesicles from the Liver after Roux-en-Y Bariatric Surgery.

ABSTRACT: Circulating extracellular vesicles are small particles enclosed by a phospholipid bilayer. Vesicles deriving directly from the cellular membrane by an active budding process retain cell origin specific proteins and RNA. These vesicles carry pathophysiological information from their parental cell and hold the potential to allow analysis of organs without the need for a biopsy. We included in our study 27 patients undergoing bariatric surgery. Hepatic extracellular vesicles were determined by flow cytometry. mRNA specific for hepatic cellular origin was determined in the extracellular vesicle fraction using qPCR. Surgery led to a massive reduction of weight and overall hepatic stress as determined by alanine transaminase (ALT), aspartate transaminase (AST) and γ-glutamyltransferase (GGT). To

SUBMITTER: Rega-Kaun G 

PROVIDER: S-EPMC6539504 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature