Contribution of spinal cord glial cells to L. amazonensis experimental infection-induced pain in BALB/c mice.

ABSTRACT:

Background

The cellular and molecular pathophysiological mecha\nisms of pain processing in neglected parasitic infections such as leishmaniasis remain unknown. The present study evaluated the participation of spinal cord glial cells in the pathophysiology of pain induced by Leishmania amazonensis infection in BALB/c mice.

Methods

Mice received intra-plantar (i.pl.) injection of L. amazonensis (1 × 10⁵) and hyperalgesia, and paw edema were evaluated bilaterally for 40 days. The levels of TNF-α and IL-1β, MPO activity, and histopathology were assessed on the 40th day. ATF3 mRNA expression was assessed in DRG cells at the 30th day post-infection. Blood TNF-α and IL-1β levels and systemic parasite burden were evaluated 5-40 days after the infection. At the 30th day po

SUBMITTER: Borghi SM 

PROVIDER: S-EPMC6540403 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature