BTF3 sustains cancer stem-like phenotype of prostate cancer via stabilization of BMI1.

ABSTRACT:

Background

Cancer stem-like traits contribute to prostate cancer (PCa) progression and metastasis. Deciphering the novel molecular mechanisms underlying stem-like traits may provide important insight for developing novel therapeutics.

Methods

Immunohistochemistry and immunofluorescence assays in prostatic tissues; gain- and loss-of-function analyses using ectopic overexpression and shRNAs in PCa cell lines; measurements of tumorigenic and stemness properties, and transcription in vitro and in vivo; transcriptional analysis in public databases.

Results

We identified that overexpression of BTF3 in PCa tissues and BTF3 expression highly correlates to stem-like traits. Cancer stem-like characteristics in PCa including self-renewal and metastatic potential were impaired by

SUBMITTER: Hu J 

PROVIDER: S-EPMC6540453 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature