Genomic analyses of two novel biofilm-degrading methicillin-resistant Staphylococcus aureus phages.

ABSTRACT:

Background

Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers represent an important etiological agent of many chronic human infections. Antibiotics and host immune responses are largely ineffective against bacteria within biofilms. Alternative actions and novel antimicrobials should be considered. In this context, the use of phages to destroy MRSA biofilms presents an innovative alternative mechanism.

Results

Twenty-five MRSA biofilm producers were used as substrates to isolate MRSA-specific phages. Despite the difficulties in obtaining an isolate of this phage, two phages (UPMK_1 and UPMK_2) were isolated. Both phages varied in their ability to produce halos around their plaques, host infectivity, one-step growth curves, and electron microscopy features.

SUBMITTER: Dakheel KH 

PROVIDER: S-EPMC6540549 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature