Harnessing ¹³C-labeled myo-inositol to interrogate inositol phosphate messengers by NMR.

ABSTRACT: Inositol poly- and pyrophosphates (InsPs and PP-InsPs) are an important group of metabolites and mediate a wide range of processes in eukaryotic cells. To elucidate the functions of these molecules, robust techniques for the characterization of inositol phosphate metabolism are required, both at the biochemical and the cellular level. Here, a new tool-set is reported, which employs uniformly ¹³C-labeled compounds ([¹³C₆]myo-inositol, [¹³C₆]InsP₅, [¹³C₆]InsP₆, and [¹³C₆]5PP-InsP₅), in combination with commonly accessible NMR technology. This approach permitted the detection and quantification of InsPs and PP-InsPs within complex mixtures and at physiolog

SUBMITTER: Harmel RK 

PROVIDER: S-EPMC6540952 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature