A Robust Method for the Purification and Characterization of Recombinant Human Histone H1 Variants.

ABSTRACT: Higher order compaction of the eukaryotic genome is key to the regulation of all DNA-templated processes, including transcription. This tightly controlled process involves the formation of mononucleosomes, the fundamental unit of chromatin, packaged into higher order architectures in an H1 linker histone-dependent process. While much work has been done to delineate the precise mechanism of this event in vitro and in vivo, major gaps still exist, primarily due to a lack of molecular tools. Specifically, there has never been a successful purification and biochemical characterization of all human H1 variants. Here we present a robust method to purify H1 and illustrate its utility in the purification of all somatic variants and one germline variant. In addition, we performed a first ever side-

SUBMITTER: Osunsade A 

PROVIDER: S-EPMC6541009 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature