Quantifying How Staining Methods Bias Measurements of Neuron Morphologies.
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ABSTRACT:
SUBMITTER: Farhoodi R
PROVIDER: S-EPMC6541099 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Quantifying How Staining Methods Bias Measurements of Neuron Morphologies.
Farhoodi Roozbeh R Lansdell Benjamin James BJ Kording Konrad Paul KP
Frontiers in neuroinformatics 20190521
The process through which neurons are labeled is a key methodological choice in measuring neuron morphology. However, little is known about how this choice may bias measurements. To quantify this bias we compare the extracted morphology of neurons collected from the same rodent species, experimental condition, gender distribution, age distribution, brain region and putative cell type, but obtained with 19 distinct staining methods. We found strong biases on measured features of morphology. These ...[more]
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