Introduction and validation of a new semi-automated method to determine sympathetic fiber density in target tissues.

ABSTRACT: In recent years, the role of sympathetic nervous fibers in chronic inflammation has become increasingly evident. At the onset of inflammation, sympathetic activity is increased in the affected tissue. However, sympathetic fibers are largely absent from chronically inflamed tissue. Apparently, there is a very dynamic relationship between sympathetic innervation and the immune system in areas of inflammation, and hence a rapid and easy method for quantification of nerve fiber density of target organs is of great value to answer potential research questions. Currently, nervous fiber densities are either determined by tedious manual counting, which is not suitable for high throughput approaches, or by expensive automated processes relying on specialized software and high-end microscopy equipme

SUBMITTER: Bleck D 

PROVIDER: S-EPMC6541301 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature