Conversion of random X-inactivation to imprinted X-inactivation by maternal PRC2.

ABSTRACT: Imprinted X-inactivation silences genes exclusively on the paternally-inherited X-chromosome and is a paradigm of transgenerational epigenetic inheritance in mammals. Here, we test the role of maternal vs. zygotic Polycomb repressive complex 2 (PRC2) protein EED in orchestrating imprinted X-inactivation in mouse embryos. In maternal-null (Eed^m-/-) but not zygotic-null (Eed^-/-) early embryos, the maternal X-chromosome ectopically induced Xist and underwent inactivation. Eed^m-/- females subsequently stochastically silenced Xist from one of the two X-chromosomes and displayed random X-inactivation. This effect was exacerbated in embryos lacking both maternal and zygotic EED (Eed^mz-/-), suggesting that zygoti

SUBMITTER: Harris C 

PROVIDER: S-EPMC6541438 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature