Understanding mechanisms of oocyte development by follicular fluid lipidomics.

ABSTRACT:

Purpose

The present study aimed to provide a non-invasive approach to studying mechanisms responsible for oocyte development.

Methods

To this end, follicular fluid (FF) from 62 patients undergoing in vitro fertilization (IVF) cycles was split into two groups depending on the pregnancy outcome: pregnant (n = 28) and non-pregnant (n = 34) groups. Data were acquired by the MALDI-TOF mass spectrometry. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were applied to the data set. A ROC curve, to predict success rate, was constructed, and the lipids were attributed.

Results

Six ions were differentially represented in FF of pregnant and non-pregnant patients, with an area under the curve of 0.962. Phosphatidic acid, pho

SUBMITTER: Montani DA 

PROVIDER: S-EPMC6541691 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature