Development of Functional Antibodies Directed to Human Dialyzable Leukocyte Extract (Transferon®).

ABSTRACT: Transferon® is an immunomodulator made of a complex mixture of peptides from human dialyzable leucocyte extracts (hDLEs). Development of surrogate antibodies directed to hDLE is an indispensable tool for studies during process control and preclinical trials. These antibodies are fundamental for different analytical approaches, such as identity test and drug quantitation, as well as to characterize its pharmacokinetic and mechanisms of action. A previous murine study showed the inability of the peptides of Transferon® to induce antibody production by themselves; therefore, in this work, two approaches were tested to increase its immunogenicity: chemical conjugation of the peptides of Transferon® to carrier proteins and the use of a rabbit model. Bioconjugates were generated with Keyhole Lim

SUBMITTER: Mellado-Sanchez G 

PROVIDER: S-EPMC6541944 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature