Androgen promotes differentiation of PLZF⁺ spermatogonia pool via indirect regulatory pattern.

ABSTRACT:

Background

Androgen plays a pivotal role in spermatogenesis, accompanying a question how androgen acts on germ cells in testis since germ cells lack of androgen receptors (AR). Promyelocytic leukemia zinc-finger (PLZF) is essential for maintenance of undifferentiated spermatogonia population which is terminologically called spermatogonia progenitor cells (SPCs).

Aims

We aim to figure out the molecular connections between androgen and fates of PLZF⁺ SPCs population.

Method

Immunohistochemistry was conducted to confirm that postnatal testicular germ cells lacked endogenous AR. Subsequently, total cells were isolated from 5 dpp (day post partum) mouse testes, and dihydrotestosterone (DHT) and/or bicalutamide treatment manifested that Plzf was indirectly regula

SUBMITTER: Wang J 

PROVIDER: S-EPMC6542041 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature