The endo-lysosomal system of bEnd.3 and hCMEC/D3 brain endothelial cells.

ABSTRACT:

Background

Brain endothelial cell-based in vitro models are among the most versatile tools in blood-brain barrier research for testing drug penetration to the central nervous system. Transcytosis of large pharmaceuticals across the brain capillary endothelium involves the complex endo-lysosomal system. This system consists of several types of vesicle, such as early, late and recycling endosomes, retromer-positive structures, and lysosomes. Since the endo-lysosomal system in endothelial cell lines of in vitro blood-brain barrier models has not been investigated in detail, our aim was to characterize this system in different models.

Methods

For the investigation, we have chosen two widely-used models for in vitro drug transport studies: the bEnd.3 mouse and the hCMEC/D3 human

SUBMITTER: Toth AE 

PROVIDER: S-EPMC6542060 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature