Clubroot resistance gene Rcr6 in Brassica nigra resides in a genomic region homologous to chromosome A08 in B. rapa.

ABSTRACT:

Background

Clubroot, caused by Plasmodiophora brassicae Woronin, is a very important disease of Brassica species. Management of clubroot relies heavily on genetic resistance. In a cross of Brassica nigra lines PI 219576 (highly resistant, R) × CR2748 (highly susceptible, S) to clubroot, all F₁ plants were resistant to clubroot. There was a 1:1 ratio of R:S in the BC₁ and 3R:1S in the F₂, which indicated that a single dominant gene controlled clubroot resistance in PI 219576. This gene was designated Rcr6. Mapping of Rcr6 was performed using genome sequencing information from A-genome of B. rapa and B-genome of B. nigra though bulked segregant RNA sequencing (BSR-Seq) and further mapping with Kompetitive Allele Specific PCR (KASP) analysis.

Result

SUBMITTER: Chang A 

PROVIDER: S-EPMC6542104 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature