Evaluation of Different Stationary Phases in the Separation of Inter-Cross-Linked Peptides.

ABSTRACT: Chemical cross-linking coupled with mass spectrometry (MS) is becoming a routinely and widely used technique for depicting and constructing protein structures and protein interaction networks. One major challenge for cross-linking/MS is the determination of informative low-abundant inter-cross-linked products, generated within a sample of high complexity. A C18 stationary phase is the conventional means for reversed-phase (RP) separation of inter-cross-linked peptides. Various RP stationary phases, which provide different selectivities and retentions, have been developed as alternatives to C18 stationary phases. In this study, two phenyl-based columns, biphenyl and fluorophenyl, were investigated and compared with a C18 phase for separating BS³ (bis(sulfosuccinimidyl)suberate) c

SUBMITTER: Fang Z 

PROVIDER: S-EPMC6542356 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature