Investigation of factors influencing the separation of diastereomers of phosphorothioated oligonucleotides.

ABSTRACT: This study presents a systematic investigation of factors influencing the chromatographic separation of diastereomers of phosphorothioated pentameric oligonucleotides as model solutes. Separation was carried out under ion-pairing conditions using an XBridge C₁₈ column. For oligonucleotides with a single sulfur substitution, the diastereomer selectivity was found to increase with decreasing carbon chain length of the tertiary alkylamine used as an ion-pair reagent. Using an ion-pair reagent with high selectivity for diastereomers, triethylammonium, it was found the selectivity increased with decreased ion-pair concentration and shallower gradient slope. Selectivity was also demonstrated to be dependent on the position of the modified linkage. Substitutions at the center of the pe

SUBMITTER: Enmark M 

PROVIDER: S-EPMC6543027 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature