Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay.

ABSTRACT:

Background

The real-time quantitative polymerase chain reaction (qPCR) is routinely used for quantification of nucleic acids and is considered the gold standard in the field of relative nucleic acid measurements. The efficiency of the qPCR reaction is one of the most important parameters in data analysis in qPCR experiments. The Minimum Information for publication of Quantitative real-time PCR Experiments (MIQE) guidelines recommends the calibration curve as the method of choice for estimation of qPCR efficiency. The precision of this method has been reported to be between SD = 0.007 (three replicates) and SD = 0.022 (no replicates).

Results

In this article, we present a novel approach to the analysis of qPCR data which has been obtained by running a dilution series. Unlike

SUBMITTER: Panina Y 

PROVIDER: S-EPMC6543629 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature