Identification of novel factors that increase enzymatic saccharification efficiency in Arabidopsis wood cells.

ABSTRACT: Developing methods to efficiently convert lignocellulosic polymers, i.e. cellulose, hemicellulose, and lignin into user-friendly carbon resources, such as fermentable sugars, is critical for improving plant biomass utilization. Here, we report the identification of genes that increase enzymatic saccharification efficiency in cultured Arabidopsis wood cells. We overexpressed a set of genes that were upregulated during the early stages of in vitro xylem vessel cell differentiation, including transcription factor and CAZYme genes, in Arabidopsis and tested their effects on enzymatic saccharification efficiency. Of the 96 transgenic seedlings sampled, 37 and 17 lines showed significant increases and decreases in glucose yields, respectively. Further analysis of 20 overexpression lines with hig

SUBMITTER: Ohtani M 

PROVIDER: S-EPMC6543695 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature