Transplantation of Mature Photoreceptors in Rodents With Retinal Degeneration.

ABSTRACT:

Purpose

To demonstrate survival and integration of mature photoreceptors transplanted with the retinal pigment epithelium (RPE).

Methods

Full-thickness retina with attached RPE was harvested from healthy adult rats. Grafts were implanted into two rat models of retinal degeneration, Royal College of Surgeons (RCS) and S334ter-3. Survival of the host and transplanted retina was monitored using optical coherence tomography (OCT) for up to 6 months. The retinal structure and synaptogenesis between the host and transplant was assessed by histology and immunohistochemistry.

Results

OCT and histology demonstrated a well-preserved photoreceptor layer with inner and outer segments, while the inner retinal layers of the transplant largely disappeared. Grafts, including RPE, sur

SUBMITTER: Lorach H 

PROVIDER: S-EPMC6543858 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature