High-throughput, label-free, single-cell photoacoustic microscopy of intratumoral metabolic heterogeneity.

ABSTRACT: Intratumoral heterogeneity, which is manifested in almost all of the hallmarks of cancer, including the significantly altered metabolic profiles of cancer cells, represents a challenge to effective cancer therapy. High-throughput measurements of the metabolism of individual cancer cells would allow direct visualization and quantification of intratumoral metabolic heterogeneity, yet the throughputs of current measurement techniques are limited to about 120 cells per hour. Here, we show that single-cell photoacoustic microscopy can reach throughputs of approximately 12,000 cells per hour by trapping single cells with blood in an oxygen-diffusion-limited high-density microwell array and by using photoacoustic imaging to measure the haemoglobin oxygen change (that is, the oxygen consumption ra

SUBMITTER: Hai P 

PROVIDER: S-EPMC6544054 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature