Intestinal epithelial replacement by transplantation of cultured murine and human cells into the small intestine.

ABSTRACT: Adult intestinal epithelial stem cells are a promising resource for treatment of intestinal epithelial disorders that cause intestinal failure and for intestinal tissue engineering. We developed two different animal models to study the implantation of cultured murine and human intestinal epithelial cells in the less differentiated "spheroid" state and the more differentiated "enteroid" state into the denuded small intestine of mice. Engraftment of donor cells could not be achieved while the recipient intestine remained in continuity. However, we were able to demonstrate successful implantation of murine and human epithelial cells when the graft segment was in a bypassed loop of jejunum. Implantation of donor cells occurred in a random fashion in villus and crypt areas. Engraftment was obse

SUBMITTER: Khalil HA 

PROVIDER: S-EPMC6544206 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature