Microfluidic arenas for war games between neutrophils and microbes.

ABSTRACT: Measurements of neutrophil activities such as cell migration and phagocytosis are generally performed using low-content bulk assays, which provide little detail activity at the single cell level, or flow cytometry methods, which have the single cell resolution but lack perspective on the kinetics of the process. Here, we present a microfluidic assay for measuring the essential functions that contribute to the antimicrobial activity of neutrophils: migration towards the target, and killing of microbes. The assay interrogates the interactions between isolated human neutrophils and populations of live, proliferating microbes. The outcome is measured in a binary mode that is reflective of in vivo infections, which are either cleared or endure the host response. The outcome of the interactions

SUBMITTER: Ellett F 

PROVIDER: S-EPMC6544356 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature