Effect of environmental salt concentration on the Helicobacter pylori exoproteome.

ABSTRACT: Helicobacter pylori infection and a high salt diet are each risk factors for gastric cancer. In this study, we tested the hypothesis that environmental salt concentration influences the composition of the H. pylori exoproteome. H. pylori was cultured in media containing varying concentrations of sodium chloride, and aliquots were fractionated and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). We identified proteins that were selectively released into the extracellular space, and we identified selectively released proteins that were differentially abundant in culture supernatants, depending on the environmental salt concentration. We also used RNA-seq analysis to identify genes that were differentially expressed in response to environmental salt concentration. The sa

SUBMITTER: Caston RR 

PROVIDER: S-EPMC6544391 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature