IFNγ induces epigenetic programming of human T-bet^hi B cells and promotes TLR7/8 and IL-21 induced differentiation.

ABSTRACT: Although B cells expressing the IFNγR or the IFNγ-inducible transcription factor T-bet promote autoimmunity in Systemic Lupus Erythematosus (SLE)-prone mouse models, the role for IFNγ signaling in human antibody responses is unknown. We show that elevated levels of IFNγ in SLE patients correlate with expansion of the T-bet expressing IgD^negCD27^negCD11c⁺CXCR5^neg (DN2) pre-antibody secreting cell (pre-ASC) subset. We demonstrate that naïve B cells form T-bet^hi pre-ASCs following stimulation with either Th1 cells or with IFNγ, IL-2, anti-Ig and TLR7/8 ligand and that IL-21 dependent ASC formation is significantly enhanced by IFNγ or IFNγ-producing T cells. IFNγ promotes ASC development by synergizing with IL-2 and TLR7/8 ligands to induce

SUBMITTER: Zumaquero E 

PROVIDER: S-EPMC6544433 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature