SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues.

ABSTRACT: Fluorescence in situ hybridization (FISH) reveals the abundance and positioning of nucleic acid sequences in fixed samples. Despite recent advances in multiplexed amplification of FISH signals, it remains challenging to achieve high levels of simultaneous amplification and sequential detection with high sampling efficiency and simple workflows. Here we introduce signal amplification by exchange reaction (SABER), which endows oligonucleotide-based FISH probes with long, single-stranded DNA concatemers that aggregate a multitude of short complementary fluorescent imager strands. We show that SABER amplified RNA and DNA FISH signals (5- to 450-fold) in fixed cells and tissues. We also applied 17 orthogonal amplifiers against chromosomal targets simultaneously and detected mRNAs with high effi

SUBMITTER: Kishi JY 

PROVIDER: S-EPMC6544483 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature