Shape and Interhelical Spacing of DNA Origami Nanostructures Studied by Small-Angle X-ray Scattering.

ABSTRACT: Scaffolded DNA origami nanostructures enable the self-assembly of arbitrarily shaped objects with unprecedented accuracy. Yet, varying physiological conditions are prone to induce slight structural changes in the nanoscale architecture. Here, we report on high precision measurements of overall shape and interhelical distance of three prototypic DNA origami structures in solution using synchrotron small-angle X-ray scattering. Sheet-, brick-, and cylinder-shaped DNA constructs were assembled and the shape factors determined with angstrom resolution from fits to the scattering profiles. With decreasing MgCl2 concentration electrostatic swelling of both shape cross section and interhelical DNA spacing of the DNA origami structures is observed. The structures tolerate up to 10% interhelical ex

SUBMITTER: Fischer S 

PROVIDER: S-EPMC6544510 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature