Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model.

ABSTRACT: Oral antibodies that interfere with gastrointestinal targets and can be manufactured at scale are needed. Here we show that a single-gene-encoded monomeric immunoglobulin A (IgA)-like antibody, composed of camelid variable single domain antibodies (VHH) fused to IgA Fc (mVHH-IgA), prevents infection by enterotoxigenic Escherichia coli (F4-ETEC) in piglets. The mVHH-IgA can be produced in soybean seeds or secreted from the yeast Pichia pastoris, freeze- or spray-dried and orally delivered within food.

SUBMITTER: Virdi V 

PROVIDER: S-EPMC6544532 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature