A basic protein, N25, from a mollusk modifies calcium carbonate morphology and shell biomineralization.

ABSTRACT: Biomineralization is a widespread biological process in the formation of shells, teeth, or bones. Matrix proteins in biominerals have been widely investigated for their roles in directing biomineralization processes such as crystal morphologies, polymorphs, and orientations. Here, we characterized a basic matrix protein, named mantle protein N25 (N25), identified previously in the Akoya pearl oyster (Pinctada fucata). Unlike some known acidic matrix proteins containing Asp or Glu as possible Ca²⁺-binding residues, we found that N25 is rich in Pro (12.4%), Ser (12.8%), and Lys (8.8%), suggesting it may perform a different function. We used the recombinant protein purified by refolding from inclusion bodies in a Ca(HCO₃)₂ supersaturation system and fou

SUBMITTER: Yang D 

PROVIDER: S-EPMC6544838 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature