Single-cell RNA sequencing reveals the impact of chromosomal instability on glioblastoma cancer stem cells.

ABSTRACT:

Background

Intra-tumor heterogeneity stems from genetic, epigenetic, functional, and environmental differences among tumor cells. A major source of genetic heterogeneity comes from DNA sequence differences and/or whole chromosome and focal copy number variations (CNVs). Whole chromosome CNVs are caused by chromosomal instability (CIN) that is defined by a persistently high rate of chromosome mis-segregation. Accordingly, CIN causes constantly changing karyotypes that result in extensive cell-to-cell genetic heterogeneity. How the genetic heterogeneity caused by CIN influences gene expression in individual cells remains unknown.

Methods

We performed single-cell RNA sequencing on a chromosomally unstable glioblastoma cancer stem cell (CSC) line and a control normal, diploid ne

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC6545015 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature